Proteins are the workhorse in biological systems facilitating most of the biological processes in a cell, including gene expression, cell growth, proliferation, nutrient uptake, intercellular communication, and apoptosis.
The blueprint for protein synthesis is stored in DNA, which serves as a template for the highly regulated transcriptional process for the production of messenger RNA (mRNA). There are many companies available that provide various recombinant protein products. To know more about recombinant proteins. you can also navigate https://www.bosterbio.com/featured-products.
The message encoded by the mRNA is then translated into the specific amino acid sequences that make up the protein. Proteins are synthesized in a similar two-step process in all organisms – DNA is first transcribed into RNA, then RNA is converted into protein.
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Recombinant proteins are proteins encoded by recombinant DNA cloned into expression vectors that support gene expression and translation of messenger RNA. Gene modification with recombinant DNA technology can cause mutated protein expression.
Recombinant protein is an engineered form of natural protein that is created in various ways to increase protein production, modify gene sequences, and create useful commercial products.
Recombinant protein production begins at the genetic level, where the coding sequence for the desired protein is first isolated and cloned into a plasmid expression vector. Most of the recombinant proteins for therapeutic use are of human origin but are expressed in culture in microorganisms such as bacteria, yeast, or animal cells.
Human genes are very complex and often contain non-coding DNA sequences known as introns. Therefore, intron-free versions of genes are often made by converting mRNA into cDNA. Since cDNA lacks regulatory regions, expression vectors provide promoters, ribosomal binding sites, and terminator sequences.
The production of recombinant proteins for research purposes is mainly based on the economy, simplicity and speed of the process in terms of sufficient product yield.